Google Play icon

Optimal timing of mesenchymal stem cells therapy for neonatal intraventricular hemorrhage

Posted October 29, 2015
This news or article is intended for readers with certain scientific or professional knowledge in the field.


We recently showed that intraventricular transplantation of human umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) significantly attenuated posthemorrhagic hydrocephalus (PHH) and brain injury after severe intraventricular hemorrhage (IVH) in newborn rat pups. The purpose of this study was to optimize the timing of MSC transplantation for severe IVH.


Severe IVH was induced by injecting 100 uL of blood into each ventricle of Sprague-Dawley rats on postnatal day 4 (P4). Human umbilical cord blood (UCB)-derived MSCs (1×105 cells in 10ul of normal saline) were transplanted intraventricularly under stereotaxic guidance either early at P6 or late at P11. Serial brain MRIs and behavioral function tests, such as negative geotaxis and rotarod tests, were performed. At P32, brain tissue samples were obtained for histological and biochemical analyses.


Intracerebroventricular transplantation of MSCs significantly attenuated the development of PHH, decreased impaired behavioral tests, increased TUNEL-positive cells, increased astrogliosis, reduced corpus callosum thickness, reduced myelin basic protein expression, and increased the inflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6, and tumor necrosis factor-α at P6 but not at P11 after induction of severe IVH.


Intracerebroventricular transplantation of human UCB-derived MSCs attenuated PHH and brain injury after severe IVH in newborn rats in a time-dependent manner. Significant neuroprotection was only demonstrated when administered early at 2 days after induction but not late at 7 days after induction of severe IVH.

Source: PubMed

Featured news from related categories:

Technology Org App
Google Play icon
84,820 science & technology articles

Most Popular Articles

  1. New Class of Painkillers Offers all the Benefits of Opioids, Minus the Side Effects and Addictiveness (2 days old)
  2. Top NASA Manager Says the 2024 Moon Landing by Astronauts might not Happen (September 19, 2019)
  3. How social media altered the good parenting ideal (September 4, 2019)
  4. What's the difference between offensive and defensive hand grenades? (September 26, 2019)
  5. Just How Feasible is a Warp Drive? (September 25, 2019)

Follow us

Facebook   Twitter   Pinterest   Tumblr   RSS   Newsletter via Email