Biochemists from Trinity College Dublin have devised a new technique that will make the difficult but critical job of blueprinting certain proteins considerably faster, easier and cheaper.
The breakthrough will make a big splash in the field of drug discovery and development, where precise protein structure blueprints can help researchers understand how individual proteins work. Critically, these blueprints can show weaknesses that allow drug developers to draw up specific battle plans in the fight against diseases and infections.
Professor of Membrane Structural and Functional Biology at Trinity, Martin Caffrey, is the senior author of the research, which has just been published in the international peer-reviewed journal Acta Crystallographica D.
Over 50% of drugs on the market target cell membrane proteins, which are vital for the everyday functioning of complex cellular processes. They act as transporters to ensure that specific molecules enter and leave our cells, as signal interpreters important in decoding messages and initiating responses, and as agents that speed up appropriate responses.
The major challenge facing researchers is the production of large membrane protein crystals, which are used to determine the precise 3-D structural blueprints. That challenge has now been lessened thanks to the Trinity biochemists’ advent – the in meso in situ serial crystallography (IMISX) method.
Beforehand, researchers needed to harvest protein crystals and cool them at inhospitable temperatures in a complex set of events that was damaging, inefficient and prone to error. The IMISX method allows researchers to determine structural blueprints as and where the crystals grow.
Source: Trinity College Dublin