Google Play icon

Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration

Share
Posted December 3, 2014
This news or article is intended for readers with certain scientific or professional knowledge in the field.

3D printing technique is the most sophisticated technique to produce scaffolds with tailorable physical properties. But, these scaffolds often suffer from limited biological functionality as they are typically made from synthetic materials. Cell-laid mineralized ECM was shown to be potential for improving the cellular responses and drive osteogenesis of stem cells.

Here, we intend to improve the biological functionality of 3D-printed synthetic scaffolds by ornamenting them with cell-laid mineralized extracellular matrix (ECM) that mimics a bony microenvironment. We developed bone graft substitutes by using 3D printed scaffolds made from a composite of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and β-tricalcium phosphate (β-TCP) and mineralized ECM laid by human nasal inferior turbinate tissue-derived mesenchymal stromal cells (hTMSCs). A rotary flask bioreactor was used to culture hTMSCs on the scaffolds to foster formation of mineralized ECM. A freeze/thaw cycle in hypotonic buffer was used to efficiently decellularize (97% DNA reduction) the ECM-ornamented scaffolds while preserving its main organic and inorganic components. The ECM-ornamented 3D printed scaffolds supported osteoblastic differentiation of newly-seeded hTMSCs by upregulating four typical osteoblastic genes (4-fold higher RUNX2; 3-fold higher ALP; 4-fold higher osteocalcin; and 4-fold higher osteopontin) and increasing calcium deposition compared to bare 3D printed scaffolds. In vivo, in ectopic and orthotopic models in rats, ECM-ornamented scaffolds induced greater bone formation than that of bare scaffolds.

These results suggest a valuable method to produce ECM-ornamented 3D printed scaffolds as off-the-shelf bone graft substitutes that combine tunable physical properties with physiological presentation of biological signals.

Source: PubMed

Featured news from related categories:

Technology Org App
Google Play icon
87,522 science & technology articles

Most Popular Articles

  1. An 18 carat gold nugget made of plastic (January 13, 2020)
  2. Anti Solar Cells: A Photovoltaic Cell That Works at Night (February 3, 2020)
  3. Toyota Raize is a new cool compact SUV that we will not see in this part of the world (November 24, 2019)
  4. Nuclear waste could be recycled for diamond battery power (January 21, 2020)
  5. Physicist Proposes a Testable Theory Stating that Information has Mass and could Account for Universe s Dark Matter (January 24, 2020)

Follow us

Facebook   Twitter   Pinterest   Tumblr   RSS   Newsletter via Email