Google Play icon

Human Periosteal Derived Stem Cell Potential: The Impact of age

Posted September 24, 2014
This news or article is intended for readers with certain scientific or professional knowledge in the field.

There is a great deal of interest in the understanding of possible age-related changes in Mesenchymal Stem Cells in view of their use for regenerative medicine applications. Given to the outmost standing of periosteum in bone biology and to probe data for a cell-based therapy promoting graft osseointegration in the elderly, we tried to identify specific aging markers or pattern of expression in human periosteal precursor cells. Immunohistochemical detection of Ki67 and p53, Nitric Oxide production and qRT- PCR of a selected gene panel for osteoblastic differentiation, bone remodeling and stemness were evaluated. We confirmed that both Ki67 and p53 are noteworthy indicators of senescence in human periosteal precursor cells and their expression significantly correlate with cell NO production. Moreover, cell age affects genes involved in bone remodeling, with a significant increase in interleukin-6 mRNA expression and receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio. The analysis of mRNAs of genes involved in pluripotency regulation and self-renewal of stem cells, evidenced changes at least in part related to bone remodeling. We believe that this is the first study taking on age-related changes in human periosteal precursor cells, and paving the way toward new regenerative medicine strategies in bone aging and/or bone metabolic diseases.

Source: PubMed

Featured news from related categories:

Technology Org App
Google Play icon
84,790 science & technology articles

Most Popular Articles

  1. Top NASA Manager Says the 2024 Moon Landing by Astronauts might not Happen (September 19, 2019)
  2. How social media altered the good parenting ideal (September 4, 2019)
  3. What's the difference between offensive and defensive hand grenades? (September 26, 2019)
  4. Just How Feasible is a Warp Drive? (September 25, 2019)
  5. NASA's Curiosity Rover Finds an Ancient Oasis on Mars (October 8, 2019)

Follow us

Facebook   Twitter   Pinterest   Tumblr   RSS   Newsletter via Email