Google Play icon

Dead body feeding larvae useful in forensic investigations

Share
Posted July 24, 2014

Non-biting blow fly Chrysomya megacephala is commonly found in dead bodies and is used in forensic investigations to determine the time of death, referred to as the post mortem interval. A report of synanthropic derived form of C. megacephala from Tamil Nadu is provided for the first time based on morphological features and molecular characterization through generation of DNA barcoding. This study, significant in forensic investigations was published in the open access Biodiversity Data Journal.

Chrysomya megacephala is commonly found in corpse in many parts of the world. It is a tremendously abundant species and is used as fish bait in northern and south-west India. It is known to breed in human faeces, decomposed meat and fish as well as in discarded organic materials.

This dipteran fly, therefore, serves as a potential vector of many diseases due to its close association with human dwellings. In general, C. megacephala is considered important in medical, veterinary and forensic sciences. Post mortem interval (PMI) determination is useful in cases of homicide, suicide and accidental or unattended death because of natural causes. An important aspect of calculating the PMI is the accurate and quick identification of the dipteran fly collected from a crime scene.

This image shows the larva and pupa of Chrysomya megacephala.

Behaviour and developmental times of dipteran flies are essential in determining the PMI. Currently, the most common way is to identify dipteran flies for examining the adult stage under a compound microscope. It requires that the larvae collected from a crime scene should be reared until development is complete. The dipteran fly can be identified in the larval form, but if the critical characteristics are small or vary ever so slightly, misidentification is possible.

Morphological identification is difficult within the dipteran flies. A more precise method is to identify the dipteran flies genetically by encoding the collected DNA sequence at mitochondrial cytochrome oxidase gene subunit I (mtCOI). Presently, three forms of C. megacephala are recognized, namely, the normal form (nf), the synanthropic derived form (sdf) and the recently reported feral derived form (fdf).

This image depicts the male and female adult ofChrysomya megacephala.

The normal form is confined to forests of South Pacific Islands while synanthropic derived form has spread around the world from Papua New Guinea. The recently reported feral derived form (fdf) in the forests of the Himalaya, India is morphologically intermediate between normal and synanthropic derived forms. However, the occurrence of the synanthropic derived form of C. megacephala has not been documented in the State of Tamil Nadu, India.

In this context, a report for the first time for C. megacephala (sdf) from Royapuram fishing harbour, Chennai, Tamil Nadu, South East India is significant. A colony of C. megacephala was established from numerous second and third instar larvae collected from decaying fishes. The life expectancy of this fly is 40-45 days. Freshly emerged adults from pupae were identified through morphological and molecular studies.

Source: Eurekalert

Featured news from related categories:

Technology Org App
Google Play icon
85,446 science & technology articles

Most Popular Articles

  1. New treatment may reverse celiac disease (October 22, 2019)
  2. "Helical Engine" Proposed by NASA Engineer could Reach 99% the Speed of Light. But could it, really? (October 17, 2019)
  3. New Class of Painkillers Offers all the Benefits of Opioids, Minus the Side Effects and Addictiveness (October 16, 2019)
  4. The World's Energy Storage Powerhouse (November 1, 2019)
  5. Plastic waste may be headed for the microwave (October 18, 2019)

Follow us

Facebook   Twitter   Pinterest   Tumblr   RSS   Newsletter via Email