Human induced pluripotent stem cells (iPSCs) have been shown to have promising capacity for stem cells therapy and tissue engineering applications. Therefore, it is essential to compare the ability of these cells with the commonly used mesenchymal stem cells (MSC) for bone tissue engineering in vitro.
Materials and Methods:
In the present study, the biological behavior and osteogenic capacity of the iPSCs were compared with MSC isolated from human adipose tissue (AT-MSC) using 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Alizarin red staining, alkaline phosphatase (ALP) activity measurements, calcium content assay and common osteogenic-related genes. Data were reported as the mean ± SD. One-way analysis of variance (ANOVA) was used to compare the results. A p-value of less than 0.05 was considered statistically significant.
According to the results, there was significant difference between the rate of proliferation of two stem cells, as iPSCs showed increased proliferation compared to AT-MSCs. During osteogenic differentiation, ALP activity and mineralization were demonstrated to be significantly higher in iPSCs. Although AT-MSC expressed higher levels of Runx2, iPSCs expressed higher amount of osteonection and osteocalcin during differentiation.
Taking together, iPSCs showed a higher capacity for osteogenic differentiation and it hold promising potential for bone tissue engineering and cell therapy applications.