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a) Key bioreactor performance data during steady-state operation from day 230 to 260 after inoculation. The arrows indicate pulse-feeding events of nitrate and ammonium (black), and methane (white). Nitrate, ammonium and methane consumption, and dinitrogen gas production, can be observed. The concentration of nitrite was negligible throughout the experiment. b, Fluorescence in situ hybridization micrograph of the bioreactor community showing the dominant ‘M. nitroreducens’ population in large, dense clusters (green), smaller flanking Kuenenia cells (magenta) and other bacteria (blue). Image source:

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