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Genetic screening identifies genes driving resistance with a guide RNA library

Posted on December 27, 2013
Genetic screening identifies genes driving resistance with a guide RNA library

Generation of a mouse genome-wide guide RNA library. Schematic of genetic screening with genome-wide guide RNA libraries. Credit: doi: 10.1038/nbt.2800
Researchers have developed a method to create a comprehensive library of mutations across all genes in the mouse genome. This library can be used to examine the role of every gene in different cell types.

CRISPR technology uses the DNA-cutting enzyme Cas9, with the help of a guide RNA sequence, to find and modify genetic targets. Scientists can easily engineer multiple new guide RNAs using standard molecular biology techniques. This makes for a much faster and efficient method to modify the genome of any cell type in any species.

The team found that a remarkable 50 of 52 guide RNAs tested successfully cut both copies of the genes. The extremely high success rate for these engineered guide RNAs seems to be consistent across many cell types, which led them to create a library of guide RNAs targeting every gene in the mouse genome.

“CRISPR technology is revolutionising how we study the behaviour of cells,” says Dr Kosuke Yusa, lead author from the Wellcome Trust Sanger Institute. “We’ve developed a thorough library that can be used by other researchers to study the role of any gene. We can create a library of this type for any cell or any species.”

Read more at: MedicalXpress

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