Microsatellites, which consist of repeating units of two to six base pairs of DNA, are desirable molecular markers because they are highly variable and co-dominant, allowing researchers to determine both parental contributions to an individual’s genotype. However, microsatellite markers are generally species-specific and frequently must be developed for each study from scratch, which can be a challenging task. Additionally, once microsatellite markers have been developed, the associated lab work is often time-consuming and expensive.
University of Cincinnati professor Theresa Culley and colleagues have established a method that increases the ease and affordability of microsatellite marker development by reducing the number of steps involved in the overall process. The new protocol is described in the October issue of Applications in Plant Sciences.
Previously, researchers would generally design primers for specific microsatellite markers and label each primer pair with fluorescent dyes in separate PCR reactions (for each individual included in the study). In studies including approximately 10 microsatellite markers and several hundred individuals, this would necessitate up to several thousand PCR reactions. The fluorescently labeled PCR products (i.e., amplified microsatellites) for each individual would then be combined, or pool-plexed, for genotyping.
Read more at: Phys.org